Related Experiment Videos
[Structure characteristics of insulin liposomes]
1Department of Pharmaceutics, School of Pharmaceutical Science, Peking University, Beijing 100083, China.
Aim:
To study the molecular location and state of insulin within insulin-lipids.
Methods:
Particle size and distribution of insulin-lipids was determined by using laser light scattering instrument, and transmission electron microscopy (TEM) was used to examine the morphology of insulin-lipids. HPLC, fluorescence scanning, digestion with trypsin and polyacrylamide gel electrophoresis was used to determine the molecular localization and state of insulin in the lipids.
Results:
The mean particle size, polydispersity and sample quality were 218.3 nm, 0.073 and 7.2 respectively. The results of HPLC determination showed insulin exist in the insulin-lipids. There was no insulin emission spectrum in the insulin-lipids fluorescence spectrum; nearly no insulin digested in the test of insulin digestion by trypsin, and no insulin band in the insulin-lipids in figure of polyacrylamide gel electrophoresis.
Conclusion:
Insulin was encapsulated within lipids.