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Related Experiment Videos

EGF signaling patterns the feather array by promoting the interbud fate.

Radhika Atit1, Ronald A Conlon, Lee Niswander

  • 1Howard Hughes Medical Institute and Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA. l-niswander@ski.mskcc.org

Developmental Cell
|February 15, 2003
PubMed
Summary

Epidermal growth factor (EGF) signaling actively promotes feather interbud identity. This signaling pathway, involving the EGF receptor (EGFR), is crucial for establishing interbud fate and patterning during feather development.

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Area of Science:

  • Developmental biology
  • Cell signaling
  • Molecular biology

Background:

  • Feather buds form in a hexagonal array, with bone morphogenetic protein (BMP) signaling inhibiting adjacent interbud tissue.
  • The specific factors promoting interbud fate and patterning remain unclear.

Purpose of the Study:

  • To investigate the role of epidermal growth factor (EGF) signaling in establishing feather interbud identity.
  • To determine if EGF signaling actively promotes interbud fate independently of BMP signaling.

Main Methods:

  • Examined expression patterns of EGF and active EGF receptor (EGFR) in interbud regions.
  • Applied exogenous EGF and EGFR inhibitors to developing feather tissues.
  • Assessed effects on epidermal proliferation, gene expression, and feather morphology.

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Main Results:

  • EGF and active EGFR are expressed in interbud regions.
  • Exogenous EGF stimulated epidermal proliferation and interbud gene expression, while reducing feather bud characteristics.
  • EGFR inhibitors led to loss of interbud fate and increased feather bud acquisition.
  • EGF signaling acts directly on the epidermis and is independent of BMP signaling.

Conclusions:

  • Epidermal growth factor (EGF) receptor (EGFR) signaling actively promotes interbud identity.
  • This EGFR-mediated pathway is essential for establishing interbud fate and patterning.
  • The developmental timing for interpreting these interbud-promoting signals is earlier than previously thought.