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Does precipitation reduce tissue staining by indocyanine green dye solutions?
Akira Nishimura1, Akira Kobayashi, Mayumi Sakurai
1Department of Ophthalmology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan.
Japanese Journal of Ophthalmology
|February 15, 2003
Summary
Precipitation of indocyanine green (ICG) dye in ophthalmic solutions like BSS Plus can weaken its staining ability on the anterior lens capsule, especially at lower concentrations. Non-precipitating solutions may offer better tissue visualization.
Area of Science:
- Ophthalmology
- Surgical Innovation
- Biomaterials Science
Background:
- Indocyanine green (ICG) is a vital dye used in ophthalmic surgery.
- ICG can precipitate when mixed with certain irrigation solutions, potentially affecting its efficacy.
- Anterior lens capsule staining is crucial for visualizing surgical planes.
Purpose of the Study:
- To investigate the impact of ICG precipitation on anterior lens capsule staining.
- To compare the staining capabilities of ICG diluted in different ophthalmic irrigation solutions.
Main Methods:
- ICG was diluted with BSS Plus, physiological saline, and Opeguard Neo.
- Precipitate formation was assessed using light microscopy.
- ICG staining of porcine anterior lens capsules was evaluated at 0.5% and 0.0625% concentrations.
Main Results:
- Precipitate formed in ICG solutions mixed with BSS Plus, but not with saline or Opeguard Neo.
- Lower ICG concentrations (0.0625%) resulted in weaker staining compared to higher concentrations (0.5%).
- Precipitation in BSS Plus minimally affected staining at 0.5% but significantly reduced it at 0.0625% concentration.
Conclusions:
- ICG precipitation can impair its staining efficacy on the anterior lens capsule and other transparent ocular tissues.
- ICG solutions that avoid precipitation may provide superior tissue staining, particularly at lower concentrations.
- The composition of irrigation solutions, such as BSS Plus, warrants consideration for ICG compatibility.