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Assembly and overexpression of membrane proteins in Escherichia coli
David Drew1, Linda Fröderberg, Louise Baars
1Department of Biochemistry and Biophysics, Stockholm University, S-106 91 Stockholm, Sweden.
Biochimica Et Biophysica Acta
|February 15, 2003
Abstract:
The bacterium Escherichia coli is one of the most popular model systems to study the assembly of membrane proteins of the so-called helix-bundle class. Here, based on this system, we review and discuss what is currently known about the assembly of these membrane proteins. In addition, we will briefly review and discuss how E. coli has been used as a vehicle for the overexpression of membrane proteins.