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Updated: Sep 27, 2026

A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens
Published on: June 7, 2017
Bacterial contamination of surgical gloves by water droplets spilt after scrubbing
J S Heal1, A W Blom, D Titcomb
1Department of Microbiology and Orthopaedic Surgery, University of Bristol, Winford Unit, Avon Orthopaedic Centre, Southmead Hospital, Bristol, UK. jimbo@gnarly99.freeserve.co.uk
Abstract:
Wound infection and deep sepsis can have disastrous consequences, particularly in orthopaedic surgery. Strict protocols, ultra-clean air, prophylactic antibiotics, and impervious gowns and drapes, have all been shown to diminish wound infection. However it remains a common and significant problem. The water droplets spilt from the surgeons hands after meticulous scrubbing with povidone iodine were cultured. The permeability of the surgical glove packaging to Gram-positive bacteria was also investigated. The water droplets from the surgeon's arms contained environmental and potentially pathogenic bacteria including a micrococcus, a coliform and coagulase-negative staphylococci. The paper packaging for the range of sterile surgical gloves tested was discovered to be permeable to Gram-positive bacteria. In conclusion accidental water droplet contamination of surgical gloves is a potential source of infection. Alternative recommendations are made.
Insights
Surgical glove packaging is permeable to bacteria, and water droplets from surgeons' hands can carry pathogens. This contamination poses a risk for wound infections in orthopaedic surgery, necessitating alternative infection control strategies.
Area of Science:
- Microbiology
- Surgical Infection Prevention
- Orthopaedic Surgery
Background:
- Wound infections and deep sepsis are significant risks in orthopaedic surgery, despite current infection control protocols.
- Existing measures like ultra-clean air, antibiotics, and protective wear reduce but do not eliminate infection rates.
Purpose of the Study:
- To investigate the potential for bacterial contamination of surgical gloves through water droplets from surgeons' hands.
- To assess the permeability of sterile surgical glove packaging to Gram-positive bacteria.
Main Methods:
- Culturing water droplets from surgeons' arms after scrubbing with povidone-iodine.
- Testing the permeability of various sterile surgical glove paper packaging to Gram-positive bacteria.
Main Results:
- Water droplets from surgeons' arms contained environmental bacteria, including micrococcus, coliforms, and coagulase-negative staphylococci.
- The paper packaging of tested sterile surgical gloves was found to be permeable to Gram-positive bacteria.
Conclusions:
- Accidental contamination of surgical gloves via water droplets is a potential source of surgical site infections.
- Current glove packaging may not provide an adequate barrier against microbial penetration.
- Alternative recommendations for preventing surgical glove contamination are suggested.
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