Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Comparative analyses of potato expressed sequence tag libraries.

Catherine M Ronning1, Svetlana S Stegalkina, Robert A Ascenzi

  • 1The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA.

Plant Physiology
|February 15, 2003
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advancements in extracellular vesicle research.

Extracellular vesicle·2026
Same author

Hidden Messages in Extracellular Vesicles: Cross-Kingdom RNA Communication in Plant and Microbe Interactions.

Annual review of cell and developmental biology·2026
Same author

Supporting a rhubarb renaissance in North America: Understanding the history, pathology, and future research needs.

Plant disease·2026
Same author

Engineering carotenoid and steroidal glycoalkaloid depleted tomato fruit for heterologous production of high value terpenes.

bioRxiv : the preprint server for biology·2026
Same author

Trans-grafting revolution: From molecular regulation mechanisms to biotech applications.

Current opinion in plant biology·2026
Same author

Evaluation of downy mildew resistance in the USDA Hemp Germplasm Repository using high-throughput and traditional phenotyping methods.

Plant disease·2026

This study surveyed the potato transcriptome, identifying unique genes involved in tuber development and late blight resistance. The findings provide a foundation for understanding potato

Area of Science:

  • Plant Biology
  • Genomics
  • Molecular Biology

Background:

  • The cultivated potato (Solanum tuberosum) exhibits unique traits within the Solanaceae family, including modified stems (stolons) forming edible tubers.
  • Understanding potato biology is crucial due to its agricultural importance.

Purpose of the Study:

  • To comprehensively survey the potato transcriptome using expressed sequence tags (ESTs) from diverse tissues.
  • To identify genes associated with key potato physiological processes like tuber development and pathogen resistance.

Main Methods:

  • Generation of 61,940 ESTs from aerial, below-ground, and late-blight-challenged potato tissues.
  • Clustering and assembly of ESTs to identify unique sequences and tentative consensus sequences.
  • Hierarchical and k-means clustering to correlate gene expression with physiological events.

Related Experiment Videos

Main Results:

  • Generated 19,892 unique sequences from 61,940 ESTs.
  • Identified functions for 43.7% of sequences, with 48 constitutively expressed and 13,068 uniquely expressed.
  • Correlated gene expression patterns with tuber initiation, dormancy, sprouting, and potato-late blight interactions.

Conclusions:

  • The study provides a foundational dataset for potato gene expression and functional genomics.
  • Identified novel and characterized sequences linked to tuber development and resistance mechanisms against Phytophthora infestans.
  • Highlights the utility of transcriptome surveys for dissecting complex plant biology.