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Related Experiment Videos

[End point and kinetics. Pluses and minuses].

N A Fedicheva, N L Parshenkov, L V Gorbov

    Klinicheskaia Laboratornaia Diagnostika
    |February 18, 2003
    PubMed
    Summary

    The end point method for measuring transaminase activity, including alanine aminotransferase (ALT) and aspartate aminotransferase (AST), fails to detect moderate increases. This limitation hinders its use in monitoring endogenous intoxication syndromes.

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    Bulletin of experimental biology and medicine·2005

    Area of Science:

    • Clinical Biochemistry
    • Laboratory Medicine

    Background:

    • Transaminase activity, including ALT and AST, is a key biomarker in clinical diagnostics.
    • Laboratory quality control is essential for reliable diagnostic testing.

    Purpose of the Study:

    • To evaluate and compare the laboratory quality control results of two methods for determining transaminase activity: end point and kinetic.
    • To assess the suitability of the end point method for monitoring conditions like endogenous intoxication.

    Main Methods:

    • Dispersion analysis was employed to compare the two methods.
    • Laboratory quality control data for ALT and AST determination were analyzed.

    Main Results:

    • Both the end point and kinetic methods align with RF Ministry of Health order # 45.
    • The end point method does not accurately register moderately elevated levels of ALT and AST.
    • The kinetic method demonstrates greater sensitivity in detecting enzyme level changes.

    Conclusions:

    • The end point method's inability to detect moderate increases in transaminase levels limits its clinical application.
    • The end point method is not recommended for verifying or monitoring endogenous intoxication syndromes.
    • The kinetic method is preferable for accurate assessment of transaminase activity in relevant clinical scenarios.

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