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Tribulosin and beta-sitosterol-D-glucoside, the anthelmintic principles of Tribulus terrestris
M Deepak1, G Dipankar, D Prashanth
1Phytochemistry Laboratory, R&D Centre, Natural Remedies Pvt. Ltd., Veerasandra Indl. Area, Bangalore, India.
Abstract:
Successive extracts of Tribulus terrestris prepared using petroleum ether, chloroform, 50% methanol and water were tested for anthelmintic activity in-vitro using the nematode Caenorhabditis elegans. The activity could be detected only in 50% methanol extract which on further bioactivity guided fractionation and chromatographic separation yielded a spirostanol type saponin, tribulosin and beta-sitosterol-D-glucoside. Both the compounds exhibited anthelmintic activity with ED50 of 76.25 and 82.50 microg/ml respectively.
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