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Updated: Sep 27, 2026

Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays
Published on: January 7, 2016
A 24-hour comparison of serum growth hormone concentrations in patients with heart failure versus healthy controls
Brett Duncan1, Niall M Moyna, Gary V Heller
1Division of Cardiology, Hartford Hospital, Hartford, Connecticut 06102-5037, USA.
Study Objective:
To compare endogenous serum growth hormone concentrations over a 24-hour period in patients with chronic heart failure (CHF) and matched controls.
Design:
Prospective, 24-hour, endogenous concentration comparison.
Setting:
Hospital research center.
Patients:
Eight evaluable patients with nonischemic dilated cardiomyopathy and 10 healthy control subjects, matched for age and sex.
Intervention:
Over a 24-hour period, blood was drawn from the study participants every 20 minutes for determination of growth hormone.
Measurements And Main Results:
For each patient, the area under the concentration-time curve from time 0-24 hours (AUC0-24), maximum concentration (Cmax), and minimum concentration (Cnadir) of growth hormone were determined. The AUC0-24 and Cmax were 74% (p < 0.05) and 62% (p < 0.05) lower in patients with CHF than in controls, respectively. The Cnadir for all participants was 0 microg/L. Variability in growth hormone concentrations over the 24 hours was considerable for all study participants.
Conclusions:
Growth hormone concentrations are suppressed over a 24-hour period in patients with CHF versus healthy controls. Variability in levels throughout the day suggests that a single point evaluation cannot be used to determine deficiency or abundance of growth hormone.
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