Related Experiment Video

Updated: Sep 27, 2026

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
11:19

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Published on: February 25, 2011

Delayed rRNA processing results in significant ribosome biogenesis and functional defects

Arturas Meskauskas1, Jennifer L Baxter, Edward A Carr

  • 1Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742, USA.

Molecular and Cellular Biology
|February 18, 2003
PubMed

Insights

The histone deacetylase RPD3 controls ribosomal frameshifting and virus stability in yeast. Its loss causes ribosome defects, increasing frameshifting and impacting virus maintenance.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • The mof6-1 mutation in Saccharomyces cerevisiae affects programmed -1 ribosomal frameshifting and killer virus maintenance.
  • Histone deacetylases play crucial roles in gene regulation and cellular processes.

Purpose of the Study:

  • To identify the gene responsible for the mof6-1 mutation.
  • To elucidate the role of histone deacetylase RPD3 in ribosomal frameshifting and virus maintenance.
  • To investigate the mechanism by which RPD3 influences ribosome function.

Main Methods:

  • Genetic analysis of the mof6-1 mutation in yeast.
  • Complementation studies using the human homolog of RPD3.
  • Analysis of rRNA processing and ribosome activity in wild-type and mutant strains.

Main Results:

  • The mof6-1 mutation is an allele of the histone deacetylase gene RPD3.
  • RPD3's deacetylase function is essential for normal ribosomal frameshifting and killer virus stability.
  • Loss of RPD3 function leads to delayed rRNA processing, producing ribosomes with altered tRNA affinities and reduced peptidyltransferase activity.
  • The human homolog of RPD3 can rescue these yeast defects.

Conclusions:

  • RPD3-mediated histone deacetylation is critical for proper ribosome biogenesis and function.
  • Defects in rRNA processing and ribosome activity underlie the increased frameshifting efficiencies observed in rpd3 mutants.
  • These findings highlight a conserved role for histone deacetylases in regulating translation fidelity and viral stability.

More Related Videos

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

Published on: December 2, 2009

Related Concept Videos

Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Translation01:37

Translation

Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.Translation Produces the Building Blocks of LifeProteins are called the...
Translation01:37

Translation

Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.Translation Produces the Building Blocks of LifeProteins are called the...
Improving Translational Accuracy02:07

Improving Translational Accuracy

Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...