Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decrease in malate dehydrogenase activities in peripheral leucocytes of type 1 diabetic dogs.

T Arai1, M Nakamura, E Magori

  • 1Department of Veterinary Science, Nippon Veterinary and Animal Science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan. tarai@nvau.ac.jp

Research in Veterinary Science
|February 19, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Helical field-driven translational-rotational conversion in conductors.

Physical review. E·2026
Same author

Malate dehydrogenase activities are lower in some types of peripheral leucocytes of dogs and cats with type 1 diabetes mellitus.

Research in veterinary science·2004
Same author

Changes in activities of enzymes related to energy metabolism in peripheral leukocytes of diabetic dogs with glycemic control by intensive insulin treatment.

Research in veterinary science·2002
Same author

[Continuous epidural infusion of bupivacaine and buprenorphine for postoperative pain relief].

Masui. The Japanese journal of anesthesiology·1992
Same author

[Sevoflurane anesthesia for asthmatic patients: report of five cases].

Masui. The Japanese journal of anesthesiology·1992
Same author

Occurrence of the nfxB type mutation in clinical isolates of Pseudomonas aeruginosa.

Antimicrobial agents and chemotherapy·1992
Same journal

Evaluating detection of Histophilus somni immunoglobulin-binding protein A DR2 Fic: A species-specific gene target for recombinase polymerase amplification relative to long-read sequencing of respiratory samples from feedlot calves.

Research in veterinary science·2026
Same journal

Evaluation of bacterial adsorption by ultrapure microporous carbon spheres in dairy cattle: From an in vitro adsorption assay to culture-based assessment of postpartum uterine bacteria.

Research in veterinary science·2026
Same journal

Diagnostic criteria for equine thoracolumbar myofascial pain syndrome: A foundational study.

Research in veterinary science·2026
Same journal

Exploring the prognostic implications of programmed death-ligand 1 expression in canine nodal lymphoma: Insights from surface membrane expression, transcript amount and plasmatic levels.

Research in veterinary science·2026
Same journal

Infrared thermography in cats with squamous cell carcinoma: A pilot study.

Research in veterinary science·2026
Same journal

Reproductive and productive performance in vaccinated and non-vaccinated dairy cows with antibodies against bluetongue virus serotype 3.

Research in veterinary science·2026
See all related articles

Peripheral leucocytes in diabetic dogs show decreased malate dehydrogenase (MDH) activity, impacting the malate-aspartate shuttle. This suggests depressed energy metabolism and a potentially useful M/L ratio marker for diabetic conditions.

Area of Science:

  • Biochemistry
  • Cellular Metabolism
  • Veterinary Medicine

Background:

  • Type 1 diabetes mellitus affects cellular energy metabolism.
  • The malate-aspartate shuttle is crucial for cellular energy production.
  • Enzyme activity in leucocytes can indicate metabolic status.

Purpose of the Study:

  • To investigate the malate-aspartate shuttle enzyme activities in diabetic dogs.
  • To assess the impact of diabetes on leucocyte energy metabolism.
  • To evaluate the M/L ratio as a potential biomarker for diabetic metabolic conditions.

Main Methods:

  • Measurement of malate dehydrogenase (MDH) and lactate dehydrogenase (LDH) activities in peripheral leucocytes.
  • Comparison of enzyme activities and M/L ratios between diabetic and control dogs.

Related Experiment Videos

  • Assessment of fasting plasma glucose levels in all subjects.

    • Main Results:

    • Diabetic dogs exhibited significantly higher fasting plasma glucose levels.
    • MDH activity was remarkably decreased in leucocytes of diabetic dogs.
    • The cytosolic MDH/LDH (M/L) ratio was significantly lower in diabetic dogs, indicating depressed leucocyte energy metabolism.

    Conclusions:

    • Decreased M/L ratio in leucocytes reflects impaired energy metabolism in diabetic dogs.
    • The M/L ratio may serve as a valuable indicator for assessing metabolic health in canine diabetes.
    • Further research can explore therapeutic interventions targeting this metabolic pathway.