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Related Experiment Videos

Progress in maize gene discovery: a project update.

China F Lunde1, Darren J Morrow, Laura M Roy

  • 1Plant Gene Expression Center, Buchanan Street, Albany, CA 94710, USA.

Functional & Integrative Genomics
|February 19, 2003
PubMed
Summary
This summary is machine-generated.

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The Maize Gene Discovery Project (MGDP) advanced maize gene discovery through extensive EST sequencing and mutant characterization. This plant genome initiative provides valuable genetic tools and data for researchers worldwide.

Area of Science:

  • Plant Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • The Maize Gene Discovery Project (MGDP) is a 5-year National Science Foundation (NSF)-funded initiative launched in 1998.
  • It involves a multidisciplinary collaboration of researchers from six universities.

Purpose of the Study:

  • To discover novel maize genes.
  • To develop tools for phenotypic characterization of maize mutants.
  • To facilitate maize gene discovery and functional genomics research.

Main Methods:

  • Expressed Sequence Tag (EST) sequencing.
  • Complementary DNA (cDNA) microarray production.
  • Utilizing the recombinant Mu1 transposon (RescueMu) for gene discovery and genomic sequencing.

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Main Results:

  • Sequenced over 120,000 maize ESTs and 70,000 RescueMu flanking sequences.
  • Catalogued phenotypes for 23,000 maize ears, 6,200 seedling families, and 4,000 adult plant families with MuDR/Mu and RescueMu insertion alleles.
  • Developed two of four planned "Unigene" microarray slides and slides for four EST libraries, consolidating over 24,000 unique sequences.

Conclusions:

  • The MGDP has generated substantial genomic and phenotypic data for maize.
  • Resources including microarray slides, EST clones, bacterial cultures, and seed are publicly available online via the MaizeGDB (ZmDB) website.
  • ZmDB serves as a central portal for maize sequence data, analysis tools (MuSeqBox, GeneSeqer, SplicePredictor), and links to other genetics resources.