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Experimental transmission of trivittatus virus (California virus group) by Aedes trivittatus
Abstract:
The mosquito, Aedes trivittatus, when fed through a membrane a trivittatus virus dosage of 10(3.4) to 10(5.5) suckling mouse LD50/0.03 ml of blood, transmitted the virus to suckling mice. Virus multiplication indicative of a biological vector occurred in this species. When Aedes vexans and A. triseriatus ingested similar doses of trivittatus virus, both the infection and transmission rates were low and virus multiplication was poor. These results, added to evidence based on virus isolations from mosqito populations in nature, indicate that A. trivittatus is the primary vector of trivittatus virus in the north central United States.
Insights
Aedes trivittatus mosquitoes are the primary carriers of trivittatus virus, efficiently transmitting it to mice. Other mosquito species showed poor infection and transmission rates, indicating Aedes trivittatus
Area of Science:
- Arbovirology
- Medical Entomology
- Vector-borne Diseases
Background:
- Trivittatus virus is an arbovirus found in the north central United States.
- Understanding the primary mosquito vectors is crucial for disease prevention and control.
- Previous studies have suggested potential vectors, but definitive identification is needed.
Purpose of the Study:
- To determine the vector competence of Aedes trivittatus for trivittatus virus.
- To compare the vector competence of Aedes trivittatus with other mosquito species, specifically Aedes vexans and Aedes triseriatus.
- To identify the primary mosquito vector responsible for trivittatus virus transmission in the region.
Main Methods:
- Mosquitoes (Aedes trivittatus, Aedes vexans, Aedes triseriatus) were fed a known dosage of trivittatus virus through a membrane feeder.
- Infection and transmission rates were assessed by attempting to infect suckling mice with blood meals from exposed mosquitoes.
- Virus multiplication within the mosquito's body was evaluated to determine biological vector capability.
Main Results:
- Aedes trivittatus efficiently transmitted trivittatus virus to suckling mice, with evidence of virus multiplication, confirming it as a biological vector.
- Aedes vexans and Aedes triseriatus exhibited low infection and transmission rates, with poor virus multiplication.
- Field isolations of trivittatus virus from mosquito populations further support the role of Aedes trivittatus.
Conclusions:
- Aedes trivittatus is confirmed as the primary biological vector of trivittatus virus.
- Aedes vexans and Aedes triseriatus play a minimal role in trivittatus virus transmission.
- These findings are critical for public health strategies targeting trivittatus virus in the north central United States.