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Identification of human dehydrodolichyl diphosphate synthase gene
Shota Endo1, Yuan-Wei Zhang, Seiji Takahashi
1Institute of Multidisciplinary Research for Advanced Materials (IMRAM), Tohoku University, Katahira 2-1-1, Aoba-ku, 980-8577, Sendai, Japan.
Biochimica Et Biophysica Acta
|February 20, 2003
Abstract:
We isolated a cDNA encoding human dehydrodolichyl diphosphate (Dedol-PP) synthase and expressed the gene in a yeast mutant strain SNH23-7D, which is deficient in Dedol-PP synthase activity. The identity of the cloned enzyme was confirmed by functional complementation of SNH23-7D strain together with in vitro Dedol-PP synthase activity assay. Northern blot analysis indicated that testis and kidney expressed Dedol-PP synthase mRNA at high levels.