Large monocytoid cell in mononuclear cell fraction of bovine blood

Sodium metrizoate-ficoll separation of bovine mononuclear cells from peripheral blood yielded a granulocyte-free leukocyte population with an average recovery of 76%. A high proportion of the separated cells were of large size and monocytoid in appearance. This cell type was observed in a much smaller percentage in mononuclear preparations separated from defibrinated blood.