Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Extending STR markers in Y chromosome haplotypes.

S Beleza1, C Alves, A González-Neira

  • 1IPATIMUP, Instituto de Patologia e Imunologia Molecular da Universidade do Porto, Rua Dr. Roberto Frias, s/n, 4200 Porto, Portugal. sbeleza@ipatimup.pt

International Journal of Legal Medicine
|February 20, 2003
PubMed
Summary

This study developed multiplex reactions for 16 Y-STRs, enhancing forensic analysis with higher haplotype diversity in a Portuguese male population sample. New alleles for GATA C4 and GATA H4 were sequenced, improving forensic casework utility.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

X-chromosomal STRs: Metapopulations and mutation rates.

Forensic science international. Genetics·2025
Same author

Elexacaftor/tezacaftor/ivacaftor, a game-changer in cystic fibrosis: The Portuguese experience.

Pulmonary pharmacology & therapeutics·2024
Same author

A dynamical measure of the black hole mass in a quasar 11 billion years ago.

Nature·2024
Same author

Spatio-temporal dynamics of phytoplankton community in a well-mixed temperate estuary (Sado Estuary, Portugal).

Scientific reports·2022
Same author

Middle eastern genetic legacy in the paternal and maternal gene pools of Chuetas.

Scientific reports·2020
Same author

Cystic fibrosis and amyotrophic lateral sclerosis, an unexpected association.

Pulmonology·2020

Area of Science:

  • Forensic Genetics
  • Population Genetics
  • Human Molecular Genetics

Background:

  • Y-chromosome Short Tandem Repeats (Y-STRs) are crucial for male lineage tracing and forensic identification.
  • Previous population studies on Y-STRs have limitations in discriminatory power.
  • Expanding the number of Y-STR loci enhances haplotype resolution.

Purpose of the Study:

  • To develop and validate multiplex reactions for 16 Y-STR loci.
  • To extend population genetic data for specific Y-STR loci (GATA A7.1, GATA A7.2, GATA C4, GATA H4) in a northern Portuguese sample.
  • To assess the forensic utility of an expanded Y-STR marker set.

Main Methods:

  • Development of two multiplex polymerase chain reactions (PCR) for amplifying 16 Y-STRs.
  • Analysis of 208 male individuals from northern Portugal.

Related Experiment Videos

  • Haplotype diversity calculations and sequence analysis of novel alleles.

    • Main Results:

    • 199 distinct haplotypes were identified using the 16 Y-STR markers.
    • Overall haplotype diversity was high (0.9996), with 190 unique haplotypes.
    • The expanded Y-STR set, particularly the 8 novel markers, demonstrated higher haplotype diversity compared to the core set.

    Conclusions:

    • The developed multiplex Y-STR systems are effective for forensic applications.
    • Inclusion of additional Y-STR markers significantly increases haplotype diversity, aiding in individual identification.
    • Sequence data for new alleles of GATA C4 and GATA H4 provide valuable information for forensic interpretation.