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Updated: Sep 27, 2026

Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation
Published on: January 22, 2020
[Influence of dibutyryl cyclic adenosine monophosphate on motility of sperm]
1Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Objectives:
To investigate the influence of cAMP/PKA signal transduction on human sperm motility, and to study the effect of dibutyryl cyclic adenosine monophosphate dibutyryl cyclic adenosine monophosphate (dbcAMP) on human sperm motility in vitro.
Methods:
Sperm aseptically obtained by masturbation and prepared by swim-up technique from 10 healthy fertile men were incubated with different concentrations of dbcAMP. Measurement of mobility were carried out at 20, 30, and 60 min in all specimens.
Results:
The sperm treated with dbcAMP showed a significant increase in sperm progressive motility and the percentage of motile cells. The effect seemed enhanced with the increasing of dbcAMP concentration. VSL and VCL were not affected by dbcAMP.
Conclusions:
dbcAMP can activate the mobility of human sperm in vitro.
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