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[Detection of DNA damage of human sperm using single cell electrophoresis]
Hai-Ou Lu1, Li Zhang, Ning Zhang
1Reproduction Centre, 202 Hospital of PLA, Shenyang, Liaoning 110003, China.
Objectives:
To detect the sperm DNA damage and to evaluate its significance in male reproductive using single cell gel electrophoresis (SCGE).
Methods:
Four hundred and eighteen sperm samples were analysed using the computer assisted analysis system and SCGE. The sperms samples were divided into five grades according to the extent of the sperm nuclear DNA damage.
Results:
1. When the sperm density is less than 20 x 10(6)/ml, the occurence of grade II and III are increased significantly; 2. In the unmotile grade d sperm the occurence of grade I comet amounts was 5.39%, the occurence of grade II and III was remarkably increased. There was a evidently variance between the grade d and grade a + b sperm.
Conclusions:
SCGE can be used to detect the sperm DNA breakage and to evaluate the sperm quality and damage.