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Published on: March 29, 2018
[The biological effects of recombinant human bone morphogenetic protein 2 on human periodontal ligament fibroblasts]
1Ninth People's Hospital, Shanghai Second Medical University.
Objective:
The purpose of this study is to study the biological effects of recombinant human bone morphogenetic protein 2 (rhBMP2) on human periodontal ligament fibroblasts (HPDLFs).
Methods:
Human periodontal ligament fibroblasts were primary cultured and detected the different doses of rhBMP2 on their proliferation, alkaline phosphatase (ALP) activity, osteocalcin (OC) synthesis and formation of the mineralized nodules.
Results:
rhBMP2 (0.25-2 mg/ml) had no remarkable effect on the proliferation of HPDLFs. The ALP activity, OC synthesis and formation of the mineralized nodules of HPDLFs were significantly stimulated by 0.5-2 mg/ml rhBMP2.
Conclusion:
The effects of rhBMP2 on HPDLFs are dose-dependent. Not only can rhBMP2 stimulate the expression but also promote the maturation of osteoblastic phenotype of HPDLFs.
