[Effects of the mechanical stress on the proliferation and function of osteoblast-like cells in vitro]

The aim of this study was to investigate the effects of the mechanical stress on the proliferation and function of osteoblast-like cells (UMR106) of rats, and further to explore the mechanism of remodeling of periodontium from an angle of mechanics.

The flow cytometry and MTT method was used to examine the number of osteoblasts-like cells and their proliferation and metabolic enzymic activity 6 h, 12 h, 18 h, 24 h, 30 h, and 36 h after the top-bottom axial stress (225 x g) was exerted on the UMR106 in vitro.

The difference of cell phase fraction and function between the experimental group and the control group were of great significance (P < 0.01). The maximum SPF increasing and functional decreasing appeared 12 h after the appliance of the stress, and all indexes were no significantly different between the two groups 24 h after the stress was supplied. Forward light scatter had no remarkable alteration.

The top-bottom axial stress (225 x g) could inevitably influence the proliferation and function of osteoblast-like cells without pathological damage occurred.