Related Experiment Video
Updated: Sep 27, 2026
Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43
Published on: September 11, 2020
[Construction of the pyruvate oxidase gene deficiency variant strain of Streptococcus oralis]
Ruoyu Pang1, Rong Zhang, Yunhui Zhang
1College of Stomatology, West China University of Medical Sciences.
Objective:
The purpose of this study is to construct a pyruvate oxidase gene deficiency variant strain of Streptococcus oralis (S. oralis).
Methods:
The sopox gene, which was got using polymerase chain reaction (PCR), and the 130-basepair segment of which was cut down with endonuclease BamHI, and transferred into S. oralis (ATCC10557) by using electrotransformation. The authors obtained a variant strain of S. oralis, and then the catalase activity of the first culture and 3-4 subcultures was examined.
Results:
The authors obtained a pyruvate oxidase gene deficiency variant strain of S. orlis. The catalase activity examination showed that the ability of producing H2 O2 of the variant strain of S. orlis declined, whose catalase activity was between those of the positive control (ATCC10557) and the negative control (Escherichia coli, JM109). But the produced H2 O2 quantity of their subcultures was less than that of the negative control.
Conclusion:
The construction of the pyruvate oxidase gene deficiency variant strain of Streptococcus oralis is successful.
