Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thyroid-stimulating monoclonal antibodies.

Jane Sanders1, Jennifer Jeffreys, Hilde Depraetere

  • 1FIRS Laboratories, RSR Ltd., Parc Ty Glas, Llanishen, Cardiff, United Kingdom.

Thyroid : Official Journal of the American Thyroid Association
|February 21, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early childhood risk of physical abuse with isolated subconjunctival hemorrhages.

Child abuse & neglect·2026
Same author

Suppressing Si Valley Excitation and Valley-Induced Spin Dephasing for Long-Distance Shuttling.

Physical review letters·2026
Same author

The thyrotropin receptor structure and interactions with autoantibodies.

European thyroid journal·2025
Same author

Meconium-related obstruction: Contemporary experience in a multi-institutional consortium.

Journal of pediatric surgery·2025
Same author

Autoantibodies to the TSH Receptor-from discovery to understanding the mechanisms of action and to new therapeutics.

Endocrine journal·2025
Same author

Safety, pharmacokinetics, and potential benefits of TSH-receptor-specific monoclonal autoantibody K1-70<sup>TM</sup> in Japanese Graves' disease patients: results of a phase 1 trial.

Endocrine journal·2025
Same journal

Stimulating Thyrotropin Receptor Antibodies Enhance the Expression of Both Thyrotropin Receptors and Insulin-Like Growth Factor 1 Receptors in Fibroblasts.

Thyroid : official journal of the American Thyroid Association·2026
Same journal

Mild Thyroid Eye Disease-The Most Common yet the Least Studied Presentation of Thyroid Eye Disease.

Thyroid : official journal of the American Thyroid Association·2026
Same journal

In Memoriam: Lawrence Crane Wood (1935-2026).

Thyroid : official journal of the American Thyroid Association·2026
Same journal

Trends in Management and Outcomes of Pediatric Hyperthyroidism Over Two Decades.

Thyroid : official journal of the American Thyroid Association·2026
Same journal

Targeted Therapy-Induced Erythrocytosis in Thyroid Cancers: An Underrecognized Safety Signal from a Retrospective Study.

Thyroid : official journal of the American Thyroid Association·2026
Same journal

<i>Retraction Notice:</i> High Iodine Concentration Attenuates <i>RET/PTC3</i> Oncogene Activation in Thyroid Follicular Cells.

Thyroid : official journal of the American Thyroid Association·2026
See all related articles

New thyrotropin (TSH) receptor monoclonal antibodies (TSHR mAbs) stimulate thyroid cells and block TSH binding. These TSHR mAbs show potential for research, diagnostics, and therapeutic applications.

Area of Science:

  • Immunology
  • Endocrinology
  • Molecular Biology

Background:

  • Thyrotropin receptor (TSHR) autoantibodies are implicated in autoimmune thyroid diseases.
  • Developing specific reagents for TSHR research and diagnostics is crucial.

Purpose of the Study:

  • To generate and characterize novel monoclonal antibodies (mAbs) targeting the TSHR.
  • To evaluate the functional and binding properties of these TSHR mAbs.

Main Methods:

  • TSHR mAbs were generated from cDNA-immunized mice.
  • Stimulation of cyclic adenosine monophosphate (cAMP) production in porcine thyroid cells was measured.
  • Inhibition of TSH binding to TSHR and binding of labeled TSmAbs to TSHR were assessed.
  • Graves' sera were used to inhibit labeled TSmAb binding.

Related Experiment Videos

Main Results:

  • Three TSHR monoclonal antibodies (TSmAbs 1-3) were identified, exhibiting distinct V(H) and V(L) sequences.
  • TSmAbs stimulated cAMP production in porcine thyroid cells and TSHR-transfected CHO cells.
  • TSmAbs inhibited TSH binding to TSHR and demonstrated high-affinity binding to TSHR.
  • Inhibition of TSmAb binding by Graves' sera correlated with TSH binding inhibition.

Conclusions:

  • TSHR mAbs possess both thyroid-stimulating and TSH-binding inhibitory activities.
  • These TSHR mAbs are valuable tools for TSHR structure-function studies.
  • TSHR mAbs can be utilized as reagents for novel TSHR autoantibody assays.
  • TSHR mAbs offer potential alternatives to recombinant TSH in in vivo applications.