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Efficient synthesis of glyceroyl beta-lactoside and its derivatives through a condensation reaction by cellulase
Nozomu Yasutake1, Kazuhide Totani, Yoichiro Harada
1Science of Biological Resource, The United Graduate School of Agricultural Science, Gifu University, Yanagido 1-1, Japan.
Abstract:
Condensation reaction between lactose and glycerol was effectively catalyzed by utilizing a commercially available cellulase preparation from Trichoderma reesei. The enzyme induced the formation of 1-O-beta-lactosyl-(R,S)-glycerol (1) and 2-O-beta-lactosyl glycerol (2) in a molar ratio of 7:3 and in a 20% yield based on lactose added. The enzyme also induced the condensation of lactose with 1,3-propanediol to produce O-beta-lactosyl propanediol (3) in a yield of 15%. When various alkanols (N: 2-8) and allyl alcohol were used in the condensation reaction, the corresponding alkyl and allyl beta-lactoside were obtained in the yields of 0.9-3.8% of the desired compounds.
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