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Human immune response to outer membrane protein CD of Moraxella catarrhalis in adults with chronic obstructive

Timothy F Murphy1, Charmaine Kirkham, Dai-Fang Liu

  • 1Division of Infectious Diseases, University at Buffalo, The State University of New York, New York, USA. murphyt@acsu.buffalo.edu

Infection and Immunity
|February 22, 2003
PubMed

Insights

Antibody responses to outer membrane protein CD were studied in adults with chronic obstructive pulmonary disease (COPD). Some patients developed new antibodies targeting this Moraxella catarrhalis protein after infection or colonization.

Area of Science:

  • Immunology
  • Microbiology
  • Pulmonology

Background:

  • Moraxella catarrhalis is a frequent cause of lower respiratory tract infections in COPD patients.
  • Outer membrane protein (OMP) CD is a conserved M. catarrhalis surface protein and a potential vaccine antigen.

Purpose of the Study:

  • To investigate the antibody response to OMP CD in adults with COPD following M. catarrhalis infection or colonization.
  • To characterize the nature and targets of these antibodies.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) to measure serum IgG.
  • Adsorption assays to identify antibodies binding to surface-exposed epitopes.
  • Immunoblot assays using fusion peptide constructs to map antibody recognition sites.
  • Measurement of mucosal IgA in sputum supernatants.

Main Results:

  • Nine of 40 patients developed new serum IgG to OMP CD post-infection/colonization.
  • In six of these patients, IgG antibodies recognized surface-exposed epitopes on OMP CD.
  • Antibodies primarily targeted conformational epitopes in the carboxy region of OMP CD.
  • Three of 28 patients developed new mucosal IgA to OMP CD in sputum.

Conclusions:

  • OMP CD elicits a systemic and mucosal immune response in some COPD patients following M. catarrhalis infection or colonization.
  • These findings support OMP CD as a potential target for vaccine development in COPD.
  • The study characterizes the immune response to a key M. catarrhalis virulence factor.

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