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Phasing at high resolution using Ta6Br12 cluster.

Sankaran Banumathi1, Miroslawa Dauter, Zbigniew Dauter

  • 1Synchrotron Radiation Research Section, Macromolecular Crystallography Laboratory, NCI, Brookhaven National Laboratory, Building 725A-X9, Upton, NY 11973, USA.

Acta Crystallographica. Section D, Biological Crystallography
|February 22, 2003
PubMed
Summary
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The tantalum-6 bromine-12 (Ta(6)Br(12)(2+)) cluster is a potent reagent for macromolecular crystallography. This cluster enables successful protein structure determination using single-wavelength anomalous diffraction (SAD) phasing.

Area of Science:

  • Crystallography
  • Structural Biology
  • Materials Science

Background:

  • The tantalum-6 bromine-12 (Ta(6)Br(12)(2+)) cluster is an effective reagent for derivatizing large macromolecules.
  • This cluster is an octahedron of six tantalum atoms with 12 bridging bromine atoms, exhibiting a spherical shape with a 6 Å radius.

Purpose of the Study:

  • To evaluate the Ta(6)Br(12)(2+) cluster as a reagent for macromolecular crystallography.
  • To assess its utility in single-wavelength anomalous diffraction (SAD) phasing.

Main Methods:

  • Derivatization of protein crystals with the Ta(6)Br(12)(2+) cluster.
  • X-ray diffraction data collection at tantalum and bromine absorption edges.
  • Single-wavelength anomalous diffraction (SAD) phasing.

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Main Results:

  • The Ta(6)Br(12)(2+) cluster provides significant anomalous diffraction signals, particularly at resolutions below 5 Å.
  • Individual tantalum atoms become resolvable beyond 3 Å, enhancing phasing power.
  • Successful high-resolution phasing of four different proteins was achieved using SAD with this cluster.

Conclusions:

  • The Ta(6)Br(12)(2+) cluster is a mild derivatization reagent.
  • Despite partial incorporation, it facilitates successful crystal structure solutions via SAD.
  • This cluster is a valuable tool for macromolecular phasing.