Related Experiment Videos
Crystallization and preliminary crystallographic studies of mung bean cytokinin-specific binding protein
Grzegorz D Bujacz1, Oliwia Pasternak, Yasuyuki Fujimoto
1Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland, and Institute of Technical Biochemistry, Technical University of Łódź, Poland.
Acta Crystallographica. Section D, Biological Crystallography
|February 22, 2003
Abstract:
Cytokinins, or plant growth hormones, bind with very high affinity to cytokinin-specific binding proteins (CSBPs). Recombinant mung bean CSBP has been overexpressed in Escherichia coli and crystallized in complex with zeatin, a natural plant growth hormone. The crystals belong to the hexagonal system, space group P6(2) or P6(4), with unit-cell parameters a = 113.62, c = 86.85 A, contain two to five copies of the protein in the asymmetric unit and diffract X-rays to 1.25 A resolution.