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Gene expression profile of mouse bone marrow stromal cells determined by cDNA microarray analysis
Georg Wieczorek1, Christine Steinhoff, Ralph Schulz
1Max-Planck-Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany.
Cell and Tissue Research
|February 22, 2003
Summary
Bone marrow stromal cells (BMSC) are multipotent adult stem cells. Gene expression analysis suggests these cells closely associate with vascular cells and may resemble pericytes.
Area of Science:
- Stem cell biology
- Mesenchymal stem cell research
Background:
- Bone marrow stromal cells (BMSC) exhibit multipotency and adult stem cell characteristics.
- Their precise identity and origin within the bone marrow remain unclear.
- BMSC can differentiate into various mesenchymal and non-mesenchymal cell types.
Purpose of the Study:
- To characterize mouse BMSC cultures using gene expression profiling.
- To investigate the cellular identity and stromal context of BMSC.
- To compare gene expression profiles between mouse and human BMSC.
Main Methods:
- Establishment and characterization of mouse BMSC cultures.
- Analysis of gene expression using cDNA microarrays.
- Comparative analysis with human BMSC expression data.
Main Results:
- High concordance in gene expression profiles between mouse and human BMSC.
- Identification of genes related to extracellular matrix, cell adhesion, cytoskeleton, and cytokines/receptors.
- Gene expression patterns indicate a close association with vascular cells.
Conclusions:
- Mouse BMSC gene expression profiles are highly comparable to human BMSC.
- BMSC gene expression suggests a close relationship with vascular cells.
- Findings indicate that BMSC may be equivalent to pericytes.