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[Preimplantation genetic diagnosis: two successful cases]
Fan Jin1, Chen-Ming Xu, Hui-Juan Gao
1The Affiliated Obstetrics and Gynecology Hospital, College of Medical Sciences, Zhejiang University, Hangzhou 310006, China.
Summary
Preimplantation genetic diagnosis technology was established, leading to two successful pregnancies in women with advanced age and tubal obstruction. This method ensures healthy fetal development through advanced embryo selection techniques.
Area of Science:
- Reproductive Medicine
- Genetics
- Embryology
Background:
- Advanced maternal age and tubal obstruction pose significant challenges to natural conception.
- Preimplantation genetic diagnosis (PGD) offers a potential solution for selecting viable embryos.
Purpose of the Study:
- To establish and validate a technology for preimplantation genetic diagnosis.
- To assess the efficacy of PGD in achieving successful pregnancies in specific patient groups.
Main Methods:
- Intracytoplasmic sperm injection (ICSI) and blastomere biopsy were utilized.
- Fluorescence in-situ hybridization (FISH) was performed on biopsied blastomeres for genetic analysis.
- Embryo transfer was conducted using genetically normal embryos.
Main Results:
- Two women underwent the procedure, with successful embryo implantation and transfer.
- Elevated serum HCG levels confirmed pregnancy establishment 20 days post-transfer.
- Ultrasonography at 16 weeks gestation indicated normal fetal growth and structure.
Conclusions:
- The established preimplantation genetic diagnosis technology is effective.
- Successful clinical pregnancies were achieved, demonstrating the viability of the PGD approach in challenging cases.