Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Advanced bioreactor with controlled application of multi-dimensional strain for tissue engineering.

Gregory H Altman1, Helen H Lu, Rebecca L Horan

  • 1Tufts University, Department of Chemical & Biological Engineering, Bioengineering Center, 4 Colby Street, Medford, MA 02155, USA.

Journal of Biomechanical Engineering
|February 25, 2003
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A bioreactor system for in vitro tendon differentiation and tendon tissue engineering.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2015
Same author

A silk-based encapsulation platform for pancreatic islet transplantation improves islet function in vivo.

Journal of tissue engineering and regenerative medicine·2015
Same author

Silk hydrogels for sustained ocular delivery of anti-vascular endothelial growth factor (anti-VEGF) therapeutics.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2015
Same author

Silk macromolecules with amino acid-poly(ethylene glycol) grafts for controlling layer-by-layer encapsulation and aggregation of recombinant bacterial cells.

ACS nano·2015
Same author

Biomineralization of stable and monodisperse vaterite microspheres using silk nanoparticles.

ACS applied materials & interfaces·2015
Same author

Programmable 3D silk bone marrow niche for platelet generation ex vivo and modeling of megakaryopoiesis pathologies.

Blood·2015

A new bioreactor system enables precise control of mechanical strain and environmental conditions for skeletal tissue engineering. This advanced system successfully supported the development of engineered anterior cruciate ligament tissue from human bone-marrow stromal cells.

Area of Science:

  • Biomedical Engineering
  • Tissue Engineering
  • Bioreactor Technology

Background:

  • Advanced bioreactors are crucial for in vitro engineering of functional skeletal tissues.
  • Current systems often lack the precise control needed for complex tissue development.
  • Developing functional anterior cruciate ligament (ACL) requires controlled mechanical and biochemical environments.

Purpose of the Study:

  • To develop and demonstrate a computer-controlled bioreactor system for skeletal tissue engineering.
  • To apply complex mechanical strains and environmental controls to three-dimensional matrices.
  • To engineer anterior cruciate ligament tissue using human bone-marrow stromal cells (hBMSCs).

Main Methods:

  • A computer-controlled, bench-top bioreactor system with 24 independent vessels was developed.

Related Experiment Videos

  • The system applied precise mechanical strains (translational and rotational) and controlled dissolved oxygen, temperature, and pH.
  • Silk fiber matrices seeded with hBMSCs were cultured for up to 21 days under controlled conditions.

    • Main Results:

    • The bioreactor system maintained precise control over mechanical strains (<0.1 micron translational, <0.1 degree rotational) and dissolved oxygen (0%-95% +/- 1%).
    • Temperature (37 +/- 0.2°C) and pH (7.4 +/- 0.02) were consistently maintained.
    • The system supported hBMSC spreading, growth, and differentiation into ligament-like cells and tissue on silk matrices, with high operational success (1/48 vessels contaminated).

    Conclusions:

    • The developed bioreactor system effectively meets the complex requirements for in vitro skeletal tissue engineering.
    • Precise control of mechanical and biochemical stimuli is essential for successful tissue development, as demonstrated with ACL engineering.
    • This technology holds significant potential for advancing tissue engineering applications, particularly for ligament reconstruction.