Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

RGS9-1 phosphorylation and Ca2+.

Theodore G Wensel1

  • 1Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA. twensel@bcm.tmc.edu

Advances in Experimental Medicine and Biology
|February 25, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential and compensatory roles for type I phosphatidylinositol-4-phosphate-5-kinase isoforms in retinal function and health.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
Same author

Structural determinants of ligand response specificity in the mast cell activating GPCR, MRGPRX2.

The Journal of biological chemistry·2026
Same author

Sub-ciliary localization of CEP290 and effects of its loss in mouse photoreceptors during development.

Journal of cell science·2025
Same author

Structural insights into brain thyroid hormone transport via MCT8 and OATP1C1.

Cell·2025
Same author

The identification of XPR1 as a voltage- and phosphate-activated phosphate-permeable ion channel.

Nature communications·2025
Same author

Roles of class III phosphatidylinositol 3-kinase, Vps34, in phagocytosis, autophagy, and endocytosis in retinal pigmented epithelium.

iScience·2025
Same journal

Peptidomics in the Spotlight: Advanced Sample Treatment Techniques and Analytical Insights.

Advances in experimental medicine and biology·2026
Same journal

Methods for the Investigation of Protein-Ligands Interactions.

Advances in experimental medicine and biology·2026
Same journal

Sample Preparation Strategies for Microbial Cell Surface Proteomics: Integrating Shaving and Shotgun Approaches.

Advances in experimental medicine and biology·2026
Same journal

Proteomic Sample Preparation for the Petroleum Industry: A Biocorrosion Case Study.

Advances in experimental medicine and biology·2026
Same journal

Proteomic and Functional Comparison of Extracellular Vesicles from Wild-Type and Lyn-Deficient Stromal Cells.

Advances in experimental medicine and biology·2026
Same journal

Proteomic Analysis of Histone Sequence Variants and Post-translationally Modified Forms.

Advances in experimental medicine and biology·2026
See all related articles

RGS9-1 phosphorylation regulates phototransduction duration in vertebrate vision. Light reduces this phosphorylation, linking calcium levels to visual signaling feedback.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Neuroscience

Background:

  • Phototransduction in vertebrate rods and cones relies on GTP hydrolysis.
  • GTPase accelerating proteins (GAPs), particularly RGS9-1, control the rate of GTP hydrolysis.
  • RGS9-1 activity is modulated by post-translational modifications like phosphorylation.

Purpose of the Study:

  • To investigate the role of RGS9-1 phosphorylation at serine 475 in regulating phototransduction.
  • To determine the impact of light and intracellular calcium (Ca2+) on RGS9-1 phosphorylation.
  • To elucidate the feedback mechanism of RGS9-1 phosphorylation in the phototransduction cascade.

Main Methods:

  • Analysis of RGS9-1 phosphorylation in vivo.
  • Measurement of intracellular Ca2+ levels in response to light.

Related Experiment Videos

  • Assessment of the effects of RGS9-1 phosphorylation on G protein and cGMP phosphodiesterase activity.

    • Main Results:

    • RGS9-1 phosphorylation at serine 475 is dependent on Ca2+.
    • Light exposure in living mice leads to a reduction in RGS9-1 phosphorylation at this site.
    • This phosphorylation state influences the duration of activated G protein and cGMP phosphodiesterase.

    Conclusions:

    • RGS9-1 phosphorylation serves as a key regulatory point in vertebrate phototransduction.
    • Light-induced changes in intracellular Ca2+ provide feedback to modulate phototransduction kinetics via RGS9-1.
    • This mechanism impacts the lifetime of key signaling molecules, fine-tuning visual responses.