Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Micropropagation of Terminalia arjuna Roxb. from cotyledonary nodes.

Seema Pandey1, V S Jaiswal

  • 1Department of Botany, Banaras Hindu University, Varanasi 221005, India.

Indian Journal of Experimental Biology
|February 25, 2003
PubMed
Summary

This study optimized the micropropagation of Terminalia arjuna using cotyledonary node explants. It achieved efficient shoot multiplication and rooting, leading to successful acclimatization and field survival of plantlets.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Models of care on the management of women with polycystic ovary syndrome: A multicentre study.

Endocrine·2026
Same author

A Prospective Randomised Comparative Clinical Trial Study of Luteal PhaseLetrozole versus Ganirelix Acetate Administration to Prevent Severity of Early Onset OHSS in ARTs.

International journal of fertility & sterility·2021
Same author

Germination and plantlet regeneration from encapsulated somatic embryos of mango (Mangifera indica L.).

Plant cell reports·2019
Same author

Plant regeneration from protoplasts of mango (Mangifera indica L.) through somatic embryogenesis.

Plant cell reports·2019
Same author

Induction of male flowers on female plants of Cannabis sativa by gibberellins and its inhibition by abscisic acid.

Planta·2014
Same author

Inhibition of gamete formation by cycloheximide in pollen tubes of Impatients balsamina.

Planta·2014

Area of Science:

  • Plant Biotechnology
  • Plant Tissue Culture
  • Horticulture

Background:

  • Terminalia arjuna is a valuable medicinal tree with significant therapeutic properties.
  • Efficient micropropagation techniques are crucial for the mass production and conservation of T. arjuna.

Purpose of the Study:

  • To develop an efficient protocol for the micropropagation of Terminalia arjuna.
  • To optimize shoot multiplication and rooting of T. arjuna using cotyledonary node explants.
  • To assess the acclimatization and field survival rates of micropropagated plantlets.

Main Methods:

  • Cotyledonary node explants from 21-day-old T. arjuna seedlings were cultured on Murashige and Skoog (MS) medium.
  • Explants were supplemented with varying concentrations of 6-benzylaminopurine (BAP) to induce shoot multiplication.

Related Experiment Videos

  • Proliferating shoot cultures were maintained through serial subculturing.
  • Rooting was induced using indole-3-butyric acid (IBA) pulse treatment.
  • Plantlets were acclimatized and transplanted to field conditions.

    • Main Results:

    • The modified MS medium with 0.5 mg/l BAP yielded a maximum of 8.9 shoots per explant.
    • Reculturing cotyledonary nodes led to a cumulative production of 45-55 shoots per explant within 60 days.
    • An 88% rooting success rate was achieved with a 15-hour pulse treatment of 1 mg/l IBA.
    • Approximately 80% of plantlets were successfully acclimatized, with 70% surviving in the field for over 6 months.

    Conclusions:

    • The developed micropropagation protocol is efficient for mass multiplication of Terminalia arjuna.
    • Optimized BAP and IBA concentrations are effective for shoot induction and rooting.
    • High acclimatization and field survival rates indicate the robustness of the protocol for commercial application and conservation efforts.