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Simple method for the quantitative examination of extra column band broadening in microchromatographic systems
Amy T Beisler1, Kathleen E Schaefer, Stephen G Weber
1Department of Chemistry, University of Pittsburgh, 219 Parkman Avenue, Pittsburgh, PA 15260, USA.
Journal of Chromatography. A
|February 25, 2003
Abstract:
In recent years capillary chromatography has gained popularity for trace analyses. Most often UV or electrochemical detection is employed because the small peak volumes make post-column derivatization challenging. We have developed a simple method based on flow injection for determining contributions to peak broadening from post-column reactors. The only requirement for application of our methodology is that diffusion be in the Taylor regime so that radial concentration gradients are relaxed enabling mixing purely by diffusion.