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Related Experiment Videos

Structure and function of the calcium pump.

David L Stokes1, N Michael Green

  • 1Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, New York 10012, USA. stokes@saturn.med.nyu.edu

Annual Review of Biophysics and Biomolecular Structure
|February 25, 2003
PubMed
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P-type ATPases, like Ca2+-ATPase, use ATP energy for ion transport via large conformational changes. Structural studies reveal how calcium binding drives these movements, linking chemical reactions to domain shifts.

Area of Science:

  • Biochemistry and Molecular Biology
  • Structural Biology
  • Membrane Transport

Background:

  • P-type ATPases are essential ATP-dependent ion pumps for active cation transport.
  • These proteins have been extensively studied for decades to understand their function.
  • Recent advances in X-ray crystallography have provided high-resolution structures of Ca2+-ATPase.

Purpose of the Study:

  • To elucidate the structural mechanisms of ATP energy coupling to calcium transport.
  • To understand how large-scale conformational changes facilitate ion translocation across membranes.
  • To correlate chemical reaction steps with specific structural alterations in P-type ATPases.

Main Methods:

  • X-ray crystallography to determine atomic structures of Ca2+-ATPase.

Related Experiment Videos

  • Comparative analysis of various structural states.
  • Correlation of structural data with existing functional and biochemical data.

    • Main Results:

    • Atomic structures reveal the detailed architecture of Ca2+-ATPase.
    • Calcium binding induces plastic deformations in the transmembrane domain.
    • Cytoplasmic domains exhibit significant rigid-body movements, facilitating substrate delivery and domain interactions.

    Conclusions:

    • Large-scale conformational changes are central to ATP-driven calcium transport.
    • Structural plasticity and domain rearrangements are key to the P-type ATPase mechanism.
    • Integration of structural and functional data provides insights into the reaction cycle of ion pumps.