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Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones
Published on: February 27, 2021
Zea mays ZmMybst1 cDNA, encodes a single Myb-repeat protein with the VASHAQKYF motif
Inderjit Singh Mercy1, Robert B Meeley, Scott E Nichols
1PO Box 5051, Agricultural University of Norway, 1432 Aas, Norway. Inderjit.mercy@bio.uio.no
Abstract:
A cDNA clone from a 4 DAP dissected maize embryo sac encoding a novel Zea mays single-repeat Myb protein is reported here. This full-length cDNA contains an ORF of 948 bp. The gene ZmMybst1 contains two introns (1166 and 706 bp) and is a single copy gene. The ZmMybst1 protein shares high sequence identity with the potato Mybst1 protein (58%). Northern blot, RT-PCR and electronic northern analysis shows that ZmMybst1 is expressed in endosperm between 4 and 30 DAP, coinciding with the period of aleurone cell differentiation and development.
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