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Updated: Aug 11, 2026
Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
Published on: September 26, 2011
Real-time quantitation of HIV-1 p24 and SIV p27 using fluorescence-linked antigen quantification assays
Matthew S Hayden1, Emil H Palacios, Robert M Grant
1Gladstone Institute of Virology and Immunology, San Francisco, CA, USA.
Abstract:
We developed fluorescence-linked antigen quantification (FLAQ) assays for HIV-1 and SIV antigen quantitation. The assays utilize polystyrene microspheres coated with monoclonal antibodies against HIV-1 Gag p24 or SIV Gag p27, which are incubated with unknown samples, flourochrome-conjugated detector antibody, and lysing agent. The fluorescence of individual microspheres is measured using flow cytometry. The speed, simplicity, and wide dynamic range of FIAQ assays makes them superior to enzyme-linked immunosorbent assays for many applications performed in research laboratories.
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