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[Study on enrichment process of total flavonoids from Euphorbia himifusa with macroporous resin]
Jixing Zhang1, Jian Li, Guohua Cheng
1Guangdong Pharmaceutical College, Guangzhou 510224.
Objective:
To study the optimal technology parameters of the enrichment process for total flavonoids of Euphorbia himifusa with macroporous resin.
Methods:
The enrichment process was studied by orthogonal experiment design using orthogonal form L9(3(4)) with the content of total flavonoids from Euphorbia himifusa as indexes, and three factors were chosen in this experiment, including the saturation of the extract sicca from Euphorbia himifusa, duration of absortion and the consistence of alcohol as eluent.
Results:
The results showed that the optimal enrichment process was that 10 ml of the extract of Euphorbia himifusa (i.e. lml 75% alcohol contains 0.5 g the extract sicca from Herba Euphorbiae Humifusae) was absorbed 30 min with a column of macroporous resin(r 15 mm x H160 mm) and the total flavonoids was eluted from the macroporous resin with 95% alcohol.
Conclusion:
The elutive rate of total flavonoids was above 93% by means of the macroporous resin. So this process enriching the total flavonoids of Euphorbia himifusa is feasible.
