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[Study on expression of beta-NGF in myoblasts transfected by PSVCEP NGF-CAT with Lipofect AMINE]
Boyong Mao1, Yanhui Liu, Junjie Chen
1Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To gain an understanding of the expression of beta-NGF in myoblasts transfected by PSVCEP NGF-CAT and the transfecting efficiency of Lipofect AMINE.
Methods:
PSVCEP NGF-CAT was delivered into cultured myoblasts by Lipofect AMINE. Expression of beta-NGF in the transfected myoblasts was detected by immunocytochemistry.
Results:
Lipofect AMINE was evidently engulfed by myoblasts at the 6th hour; about 40% myoblasts could be detected with the expression of beta-NGF by immunocytochemistry at the 48th hour.
Conclusion:
PSVCEP NGF-CAT can express beta-NGF in cultured myoblasts, and Lipofect AMINE is a convenient vector for gene transfection with simplicity and efficacy.