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Updated: Aug 10, 2026
Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
Published on: June 22, 2016
[Generation of dendritic cells from healthy human peripheral blood]
1Department of Imunology, West China School of Precilical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Objective:
To get high quality and sufficient numbers of mature dendritic cells (DC) from healthy human peripheral blood.
Methods:
Cultured plastic-adherent monocytes were isolated from healthy human peripheral blood by use of granulocyte-monocyte clony-stimulating factor and Interleukin-4 for 7 days without fed with fresh medium and cytokine.
Results:
A large number of DCs with high purity were generated. These DCs expressed HLA-I, II molecules, Costimulating molecules and adherent molecules highly, showing the characteristics of mature DC. These DCs could stimulate proliferation of allogenic T lymphocytes and had active endocytosis ability which peaked at the third day in culture but decreased afterwards.
Conclusion:
These results provide evidence that CD monocytes isolated from peripheral blood monocytes exhibit dendritic cells characteristics and may facilitate further studies of DC and its clinical application.
