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IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation
Published on: May 1, 2011
[Determination of IgG content of IVIG by improved rocket immune electrophoresis]
Fengqiong Huang1, Xiaodong Chen, Kexi Li
1Chengdu Institute of Biological Products, Chengdu 610063, China.
Objective:
To establish a specific method for determination of IgG in IVIG.
Methods:
The IVIG samples were disposed by acylation and analyzed by Rocket Immunoelectrophoresis. The amount of the IgG in IVIG was calculated by an IgG standard curve.
Results:
The standard curve by this method has a very good linear relationship: r = 0.9967. The reproducibilities with 3 bathes of IVIG samples (n = 6) are 5.37% +/- 0.21%, 5.28% +/- 0.39% and 5.80% +/- 0.30%.
Conclusion:
This method could be adapted to regular assay in blood products.
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