Interactions of neomycin and calcium in synaptosomal membranes and polyphosphoinostide monolayers

Neomycin and related aminoglycosidic antibiotics displace calcium from synaptosomes of guinea pig cerebral cortex and from preparations of phosphatidylinositol diphosphate. At low drug concentrations, inhibition of synaptosomal calcium binding is competitive (Ki = 3-10(-5) M), at high concentrations it is non-competitive (Ki = 4-10(-4) M). Monomolecular films of phosphatidylinositol diphosphate are contracted by low concentrations of neomycin in the subphase, and are expanded at high concentrations. This expansion perists even at the collapse pressure indicating a strong interaction between the drug and the lipid.