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Effect of BCG vaccination on cytokine mRNA expression in atopic children with asthma
Ayse Ozer1, Faruk Tükenmez, Anil Biricik
1Department of Medical Biology, School of Medicine, Marmara University, Haydarpasa, 81326 Istanbul, Turkey. aozer@superonline.com
Background:
To investigate whether a preexisting T(H2)-type immune response could be suppressed by BCG immunization in atopic children with asthma.
Methods And Results:
We have used PCR to amplify reverse transcribed (RT) IFN-gamma and IL-5 mRNA expressed by peripheral blood mononuclear cells (PBMCs) in response to in vitro phytohemagglutinin A, purified protein derivative and Dermatophagoides pteronyssinus II stimulation from nine atopic children, both before and 8 weeks after BCG vaccination. We have demonstrated that IFN-gamma expression was induced in response to all stimulants (IFN-gamma/beta-actin) after the vaccination, whereas there was no expression before (P<0.001). Although there was a tendency to diminish in the expression of IL-5 mRNA in response to the stimulants, only PHA rendered a statistically significant decrease after the vaccination.
Conclusions:
These results provide some evidence of TH1 dominance after BCG administration in atopic children.
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