Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Enzyme Inhibition01:30

Enzyme Inhibition

92.8K
Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
92.8K
Enzymes02:34

Enzymes

95.1K
Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
95.1K
Enzyme Kinetics01:19

Enzyme Kinetics

104.3K
Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
104.3K
Enzyme-linked Receptors01:00

Enzyme-linked Receptors

86.8K
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
86.8K
Introduction to Enzymes01:22

Introduction to Enzymes

32.4K
The use of enzymes by humans dates to 7000 BCE. Humans first used enzymes to ferment sugars and produce alcohol without knowing that this was an enzyme-catalyzed reaction. Wilhelm Kuhne coined the term 'enzyme' in 1877 from the Greek words ‘en’ meaning ‘in’ or ‘within’ and ‘zyme’ meaning ‘yeast.’
Most enzymes are proteins that speed up biochemical reactions without being consumed. Enzymes contain one or more active sites that...
32.4K
Restriction Enzymes01:11

Restriction Enzymes

36.3K
Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
36.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Costs and management of patients with hemophilia A in France: the Hemraude study.

The European journal of health economics : HEPAC : health economics in prevention and care·2021
Same author

Corrigendum to "Effect of double-filtration plasmapheresis for antibody-mediated rejection on hemostasis parameters and thrombin generation" [Thromb. Res. 166 (2018) 113-121].

Thrombosis research·2018
Same author

Effect of double-filtration plasmapheresis for antibody-mediated rejection on hemostasis parameters and thrombin generation.

Thrombosis research·2018
Same author

Comparison of the ecarin chromogenic assay and diluted thrombin time for quantification of dabigatran concentrations: comment.

Journal of thrombosis and haemostasis : JTH·2018
Same author

Impact of four direct oral anticoagulants on rotational thromboelastometry (ROTEM).

International journal of laboratory hematology·2017
Same author

Influence of Breed Size, Age, Fecal Quality, and Enteropathogen Shedding on Fecal Calprotectin and Immunoglobulin A Concentrations in Puppies During the Weaning Period.

Journal of veterinary internal medicine·2016

Related Experiment Video

Updated: Feb 11, 2026

Author Spotlight: Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus
06:45

Author Spotlight: Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

Published on: June 2, 2023

2.8K

[Thrombin: a multifunctional enzyme].

B Polack1

  • 1Service d'hématologie biologique, CHU de Grenoble, BP 217, 38043 Grenoble cedex 9. BPolack@chu-grenoble.fr

Annales De Biologie Clinique
|February 27, 2003
PubMed
Summary
This summary is machine-generated.

Thrombin, a key enzyme in blood clotting, regulates coagulation through its unique active site. Understanding its multi-functionality is crucial for developing new anticoagulant therapies.

More Related Videos

A Structured Rehabilitation Protocol for Improved Multifunctional Prosthetic Control: A Case Study
06:58

A Structured Rehabilitation Protocol for Improved Multifunctional Prosthetic Control: A Case Study

Published on: November 6, 2015

10.3K
RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
18:30

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

Published on: February 13, 2013

22.4K

Related Experiment Videos

Last Updated: Feb 11, 2026

Author Spotlight: Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus
06:45

Author Spotlight: Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

Published on: June 2, 2023

2.8K
A Structured Rehabilitation Protocol for Improved Multifunctional Prosthetic Control: A Case Study
06:58

A Structured Rehabilitation Protocol for Improved Multifunctional Prosthetic Control: A Case Study

Published on: November 6, 2015

10.3K
RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
18:30

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

Published on: February 13, 2013

22.4K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Hematology

Context:

  • Thrombin is the terminal enzyme in the blood coagulation cascade.
  • It belongs to the trypsin family of serine proteases.
  • Thrombin plays a central role in hemostasis and thrombosis.

Purpose:

  • To elucidate the structural basis of thrombin's multifunctional regulatory roles in blood coagulation.
  • To understand how active site conformation dictates substrate specificity.
  • To provide insights for the development of targeted anticoagulant therapies.

Summary:

  • Thrombin executes critical functions including fibrinogen cleavage to fibrin and platelet activation.
  • It acts as both a procoagulant (activating factors V and VIII) and an anticoagulant (activating protein C and TAFI).
  • This complex activity is attributed to the specific depth and shape of its active site, enabling precise substrate selection.

Impact:

  • Provides a mechanistic understanding of thrombin's dual pro- and anticoagulant functions.
  • Highlights the importance of active site conformation in enzyme specificity.
  • Informs the design of novel anticoagulant drugs targeting thrombin's specific activities.