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Updated: Sep 27, 2026
Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
Published on: November 13, 2017
A quantitative diffraction-based sandwich immunoassay
Jane B Goh1, Pui L Tam, Richard W Loo
1Axela Biosensors, Inc., 8th Floor, 1 Richmond Street West, Toronto, Canada M5H 3W4.
It is shown that diffraction-based sensing can be enhanced for diagnostic purposes through the use of a secondary label. The limit of detection for anti-rabbit IgG was reduced more than 40-fold by using a gold-conjugated secondary antibody. The response to secondary antibody binding was linear for concentrations from 25 to 500 ng/ml of anti-rabbit IgG, suggesting that quantitative determinations can be readily done. Moreover, the binding of the secondary antibody was observed as soon as 1 min after its introduction to the surface-bound primary complex.
It is shown that diffraction-based sensing can be enhanced for diagnostic purposes through the use of a secondary label. The limit of detection for anti-rabbit IgG was reduced more than 40-fold by using a gold-conjugated secondary antibody. The response to secondary antibody binding was linear for concentrations from 25 to 500 ng/ml of anti-rabbit IgG, suggesting that quantitative determinations can be readily done. Moreover, the binding of the secondary antibody was observed as soon as 1 min after its introduction to the surface-bound primary complex.
