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Related Experiment Videos

Functional interaction between Cydia pomonella granulovirus IAP proteins.

Ll Vilaplana1, D R O'Reilly

  • 1Department of Biological Sciences, Imperial College of Science, Technology and Medicine, Imperial College Road, London SW7 2AZ, UK. lv@wpo.nerc.ac.uk

Virus Research
|February 28, 2003
PubMed
Summary

Cydia pomonella granulovirus (CpGV) encodes three inhibitor of apoptosis (IAP) proteins. CpIAP5 enhances CpIAP3

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Area of Science:

  • Molecular biology
  • Virology
  • Insect pathology

Background:

  • Baculoviruses encode inhibitor of apoptosis (IAP) proteins to regulate host cell death.
  • Cydia pomonella granulovirus (CpGV) encodes three IAPs: CpIAP3, Cp94, and CpIAP5.
  • CpIAP3 can substitute for the p35 gene in blocking Autographa californica nucleopolyhedrovirus (AcMNPV)-induced apoptosis.

Purpose of the Study:

  • To investigate the functions of CpGV IAP proteins Cp94 and CpIAP5.
  • To determine if Cp94 and CpIAP5 interact functionally with CpIAP3.

Main Methods:

  • Transient expression experiments were performed.
  • Apoptosis was induced using actinomycin D.
  • The ability of CpGV IAPs to block apoptosis was assessed.

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Main Results:

  • Cp94 and CpIAP5, individually or together, did not block actinomycin D-induced apoptosis.
  • CpIAP5 significantly enhanced the anti-apoptotic activity of CpIAP3.
  • This study provides the first evidence of functional interactions between IAPs from a single baculovirus.

Conclusions:

  • CpGV IAP proteins Cp94 and CpIAP5 have distinct functions.
  • CpIAP5 plays a crucial role in modulating CpIAP3's anti-apoptotic activity.
  • Functional interactions among baculoviral IAPs encoded by the same virus are possible.