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Characterization of the A673 cell line (Ewing tumor) by molecular cytogenetic techniques
A Martínez-Ramírez1, S Rodríguez-Perales, B Meléndez
1Department of Human Genetics, Molecular Pathology Program, Spanish National Cancer Center (CNIO), Instituto de Salud Carlos III, Madrid, Spain. angmartinez@cnio.es
Abstract:
The A673 cell line was established from a patient with a primary rhabdomyosarcoma (RMS), which is referred to in the literature either as a Ewing tumor (ET) or as RMS. Although the two tumoral types are associated with specific and well-characterized translocations, no cytogenetic report on this cell line has been published. We characterized the A673 cell line using a combination of spectral karyotyping (SKY), fluorescence in situ hybridization (FISH), and reverse transcriptase polymerase chain reaction (RT-PCR), which revealed the presence of a complex karyotype and a translocation involving chromosomes 11 and 22 and the fusion of EWS and FLI1 genes, both events being specific to ET. Neither cytogenetics nor molecular alterations specific to RMS were found.
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