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Related Experiment Videos

Type II and type IX collagen transcript isoforms are expressed during mouse testis development.

Peter J McClive1, Andrew H Sinclair

  • 1Murdoch Children's Research Institute and Department of Paediatrics, University of Melbourne, Royal Children's Hospital, Victoria 3052, Australia. mcclivep@cryptic.rch.unimelb.edu.au

Biology of Reproduction
|February 28, 2003
PubMed
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SOX9 mutations cause campomelic dysplasia. This study reveals SOX9 regulates nonchondrocytic collagen genes in developing mouse testes, potentially impacting male gonad development and differentiation.

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • SOX9 is a transcription factor crucial for skeletal development and male sex determination.
  • Mutations in SOX9 lead to campomelic dysplasia and XY sex reversal.
  • SOX9 targets include collagen genes, but its role in non-skeletal differentiation is less understood.

Purpose of the Study:

  • To investigate SOX9's role in regulating collagen gene expression in the developing male mouse gonad.
  • To identify specific collagen transcript isoforms expressed in early gonadal development.
  • To determine if these collagen genes are expressed in Sertoli cells.

Main Methods:

  • Differential gene expression analysis.
  • Reverse transcription-polymerase chain reaction (RT-PCR).

Related Experiment Videos

  • Whole-mount in situ hybridization.
  • Analysis of testes depleted of germ cells using busulfan.

    • Main Results:

    • Identified nonchondrocytic collagen transcript isoforms (Col2a1, Col9a1, Col9a2, Col9a3) in the early male mouse gonad.
    • Male-specific gonadal expression of Col2a1 was observed from 11.5 to 13.5 days postcoitum.
    • Col9a1, Col9a2, and Col9a3 expression increased from 11.5 days postcoitum.
    • Col2a1 and Col9a3 were found to be expressed in Sertoli cells within developing testis cords.

    Conclusions:

    • SOX9 regulates nonchondrocytic collagen genes in Sertoli cells of the developing testis.
    • Nonchondrocytic type II collagen may interact with TGF-beta signaling molecules.
    • These interactions suggest a role for nonchondrocytic collagen in testis morphogenesis and differentiation.