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Related Experiment Videos

Transcription cooperation by NFAT.C/EBP composite enhancer complex.

Teddy T C Yang1, Chi-Wing Chow

  • 1Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.

The Journal of Biological Chemistry
|February 28, 2003
PubMed
Summary

Nuclear Factor of Activated T cells (NFAT) interacts with CCAAT/enhancer-binding protein (C/EBP) to form a novel composite enhancer. This NFAT.C/EBP complex potentiates gene expression, revealing a new regulatory mechanism for transcription.

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Area of Science:

  • Molecular Biology
  • Immunology
  • Genetics

Background:

  • Transcription factors regulate gene expression in various cell types.
  • Nuclear Factor of Activated T cells (NFAT) is a key transcription factor family.
  • NFAT-mediated transcription involves composite regulatory elements.

Purpose of the Study:

  • To investigate the interaction between NFAT and CCAAT/enhancer-binding protein (C/EBP).
  • To identify novel regulatory elements in gene transcription.
  • To understand the mechanism of NFAT-mediated potentiation of gene expression.

Main Methods:

  • Demonstrated NFAT and C/EBP interaction.
  • Identified formation of a ternary NFAT.C/EBP.DNA complex.
  • Analyzed regulatory regions of target genes.

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Main Results:

  • NFAT and C/EBP form a composite enhancer complex.
  • This complex potentiates peroxisome proliferator-activated receptor-gamma2 gene expression.
  • Similar NFAT.C/EBP elements are found in other gene regulatory regions.

Conclusions:

  • The NFAT.C/EBP composite element is a novel regulatory enhancer.
  • This element directs NFAT-mediated gene transcription.
  • This finding expands the understanding of transcriptional regulation by NFAT.