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Determination of proteins with Fast Red VR by a corrected resonance light-scattering technique
Chuan Xiao Yang1, Yuan Fang Li, Cheng Zhi Huang
1Institute of Environmental Chemistry, College of Chemistry and Chemical Engineering, Southwest Normal University, Chongqing 400715, The People's Republic of China.
Abstract:
A simple corrected resonance light-scattering (CRLS) technique was established to correct for any distortion of the resonance light scattering (RLS) spectra resulting from molecular absorption. By using an absorption cell holder to change the propagation direction of the incident light beam of a common spectrofluorometer, the molecular absorption was directly measured through a spectrofluorometer. With measurements of the CRLS signals of the interaction of Fast Red VR (FRV) and proteins, we proved that the present correction for the RLS spectra in terms of the molecular absorption of excitation and scattering radiation can improve the detection sensitivity by about two fold.