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Updated: Sep 27, 2026

Pull-down of Calmodulin-binding Proteins
Published on: January 23, 2012
Calmodulin inhibitors from Leucophyllum ambiguum
Susana Rojas1, Laura Acevedo, Martha Macías
1Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, D.F. 04510, México.
Abstract:
Activity-directed fractionation of a CH(2)Cl(2)-MeOH (1:1) extract of Leucophyllum ambiguum led to the isolation of two new lignans designated with the trivial names of 2'-methoxykobusin (1) and 2'-methoxy-4' '-hydroxydemethoxykobusin (2). In addition, the known compounds kobusin (3), 2',2' '-dimethoxysesamin (4), trans-cinnamic acid, apigenin, and apigetrin were obtained. The identification of the novel analogues 1 and 2 was accomplished by spectral methods. The structure of 1 was unequivocally confirmed by X-ray analysis. Compounds 1-4 interacted with bovine-brain calmodulin and inhibited the activation of the calmodulin-dependent enzyme cAMP phosphodiesterase.
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