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Published on: January 20, 2019
A sensitive bioassay for chicken interleukin-18 based on the inducible release of preformed interferon-gamma
Florian Puehler1, Thomas Göbel, Ulrike Breyer
1Department of Virology, University of Freiburg, Hermann-Herder-Str. 11, 79104, Freiburg, Germany.
Journal of Immunological Methods
|March 1, 2003
Abstract:
Conventional tests for measuring the biological activity of chicken interleukin (IL)-18 require primary chicken spleen cells. We now describe a sensitive bioassay that is based on interleukin-18-induced release of interferon (IFN)-gamma by a permanent chicken cell line. In B19-2D8 cells, cytoplasmically stored interferon-gamma is quickly secreted in response to interleukin-18 exposure.
